present and past
TRENDING NOW IN THE LAB
Read abstract excerpts from current research in glial neurobiology to be presented at SFN 2015 on Sunday Oct 8 (128.25/C60; V. B. Knight and E. E. Serrano).
Tridimensional culture induces neuronal differentiation in a commercial normal human astrocyte cell line
Tridimensional environments modulate the fate and morphology of many different cell types. When a primary human cell line derived from embryonic brain tissue was cultured in 3D (Normal Human Astrocytes; NHA; Lonza), its morphology resembled that of mixed neuronal-glial cultures. The characteristic groupings of phase-bright neuronal somata were not visible in monolayer culture where instead, cells assumed the flattened uniform appearance of glial cultures. Next Generation Sequencing technology was used to explore the genetic basis for the observed structural differences. ........... Supported by NIH P50GM068762; NMSU Manasse Endowment.